GSTZ1 expression and activity with DCA in cytosol and mitochondria of individuals with various haplotypes
Within groups of GSTZ1A carriers or noncarriers, no statistical difference was observed between cytosol and mitochondria for the ratio of activity to expression.
| Age | GSTZ1 Haplotype | Cytosol | Mitochondria | |||||
|---|---|---|---|---|---|---|---|---|
| Activity | Expression | Ratio of Act to Exp | Activity | Expression | Ratio of Act to Exp | |||
| years | pmol · min−1 · mg−1 | pmol GSTZ1/mg cytosol | pmol · min−1 · mg−1 | pmol GSTZ1/mg mitochondria | ||||
| HL735 | 74 | 1C/1C | 385 | 18.18 | 21.2 | 141 | 8.53 | 16.5 |
| HL733 | 70 | 1C/1B | 525 | 20.47 | 25.6 | 207 | 7.94 | 26.1 |
| HL750 | 10 | 1C/1B | 572 | 28.39 | 20.1 | 304 | 11.42 | 26.6 |
| HL20 | 67 | 1D/1D | 474 | 22.66 | 20.9 | 157 | 9.83 | 16.0 |
| Mean ± S.D. (n = 4) | 22.0 ± 2.5 | 21.3 ± 5.8 | ||||||
| HL10 | 23 | 1A/1C | 1238 | 18.67 | 66.3 | 871 | 12.63 | 68.9 |
| HL97 | 21 | 1A/1C | 1460 | 22.92 | 63.7 | 465 | 9.19 | 50.6 |
| HL742 | 48 | 1A/1C | 1503 | 25.18 | 59.7 | 514 | 9.87 | 52.1 |
| Mean ± S.D. (n = 3) | 63.2 ± 3.4** | 57.2 ± 10.2* | ||||||
Act, activity; Exp, expression.
↵* p < 0.05 and ** p < 0.001, significantly different from noncarriers of GSTZ1A by two-tailed t test assuming unequal variances.