Enzyme | BSA Conc. | Km, u | Vmax | CLint, u |
---|---|---|---|---|
% | μM | nmol · min−1 · mg protein−1 | μl · min−1 · mg protein−1 | |
HLM | 0 | 216 ± 32 | 3.75 ± 0.32 | 17.4 |
0.1 | 17.7 ± 1.5 | 3.25 ± 0.07 | 184 | |
0.5 | 17.7 ± 0.9 | 3.25 ± 0.05 | 184 | |
2 | 17.6 ± 1.0 | 3.14 ± 0.06 | 178 | |
UGT1A9 | 0 | 35.5 ± 2.1 | 4.90 ± 0.08 | 138 |
0.1 | 22.4 ± 1.2 | 5.59 ± 0.08 | 250 | |
0.5 | 22.7 ± 1.2 | 5.83 ± 0.09 | 257 | |
2 | 18.3 ± 1.3 | 5.79 ± 0.15 | 316 |