TABLE 3 Mechanism-based inactivation effects of TAA in P450s in vitro
TAA (0, 5, 50, 200, and 400 μM) was incubated with normal rat liver microsomes containing a NADPH regenerating system; the remaining enzyme activity was then determined by a typical dilution method. KI and kinact values were calculated from the Kitz-Wilson plot; kinact/KI values were calculated to indicate the inactivation rate.