Sequence of primers used in this study
| Primer | Sequence |
|---|---|
| For real-time RT-PCR | |
| rAADAC-RT-F | 5′-CAGAAGGAAACATGGGAAGAAC-3′ |
| rAADAC-RT-R | 5′-CCATTTTATAGATTTTGCTACAG-3' |
| mAADAC-RT-F | 5′-CAGAAGGAAACATGGGGAAA-3′ |
| mAADAC-RT-R | 5′-CATTTTGCAGGTTTTGTTACAG-3′ |
| For construction of expression plasmid | |
| pFastBac1-F | 5′-TATTCCGGATTATTCATACC-3′ |
| hHis-AADAC-R | 5′-CCCCCCGTCGACCTAGTGGTGGTGGTGGTGTAGATTTTCCTTTAGCCACT-3′ |
| rAADAC-F | 5′-GAAGATGTATATCATAGTTTACG-3′ |
| rAADAC-R | 5′-GGCTGAATTCCACAGGTACA-3′ |
| rHis-AADAC-F | 5′-CCTAGAAGAGTTGGTGTGTC-3′ |
| rHis-AADAC-R | 5′-CCCGGTACCCTAGTGGTGGTGGTGGTGCAGATTTTTGTGAAGCCAAT-3′ |
| mAADAC-F | 5′-CATCTCTGTGGTGCTTGTAG-3′ |
| mAADAC-R | 5′-ACTCATAAGTAACTGTATCGT-3′ |
| mHis-AADAC-F | 5′-GCTTAGCCCCAAAACACCAT-3′ |
| mHis-AADAC-R | 5′-CCCGGTACCTTAGTGGTGGTGGTGGTGCAGATTTTTGATAAGCCAAC-3′ |
h, human; r, Sprague-Dawley rat; m, C57BL/6J mouse; F, forward primer; R, reverse primer.