TABLE 4

Relative abundance of XMEs, transporters, nuclear receptors, and transcription factors expressed in colon cell lines and transverse colon biopsy samples

Percentages are expressed compared with transverse colon expression value [for each gene transverse colon expression is baseline (= 100%)]. Not represented in this table are 106 genes that are not expressed in transverse colon biopsy samples (for further details, see Supplemental Table 3).

Assay Identification NumberGene NameExpression Valuea in TCCaco-2Caco-2 D14C2BBe1FHCHT29HT29 D14T84
%
Phase I enzymes
    Hs00175631_m1ABP12384<5<5<5N.D.<516<5
    Hs00605175_m1ADH1B224N.D.<5N.D.N.D.N.D.N.D.N.D.
    Hs00817827_m1ADH1C2688<5<5N.D.N.D.<5N.D.<5
    Hs00923466_m1ADH4955357113N.D.N.D.N.D.N.D.
    Hs00605185_m1ADH512322513528205721
    Hs00167423_m1ADH670262516N.D.<57<5
    Hs00329084_m1ADHFE130N.D.N.D.N.D.49N.D.N.D.N.D.
    Hs00195992_m1AKR1A166034363261278843
    Hs00739326_m1AKR1B127013278227454N.D.N.D.6
    Hs00252524_m1AKR1B101400<5<5<5<5239<5
    Hs00413886_m1AKR1C1; AKR1C252284236628457506414
    Hs00366267_m1AKR1C31091459511<5146310
    Hs00230170_m1AKR1E255420785436701675145
    Hs00761005_s1AKR7A21816160103894815042
    Hs00792041_gHAKR7A3909<5<5<5<5147519
    Hs00292269_m1ALDH16A1315232941415611268
    Hs00913261_m1ALDH18A1141590766542266745
    Hs00167445_m1ALDH1A138010827294688145313139
    Hs00167476_m1ALDH1A33219622816772585
    Hs00377718_m1ALDH1B116069416677221764
    Hs00201836_m1ALDH1L1137<57N.D.N.D.<514N.D.
    Hs00355914_m1ALDH220592319262663436
    Hs00964880_m1ALDH3A176301236<5541663
    Hs00166066_m1ALDH3A2107832214613222538
    Hs00167488_m1ALDH3B18288302835315659933
    Hs00186689_m1ALDH4A1381858019318733730989
    Hs00153566_m1ALDH5A1551431052121394247
    Hs00194421_m1ALDH6A13643761333062525
    Hs00609622_m1ALDH7A141013714316186247762
    Hs00355924_m1ALDH9A142034604170232759
    Hs00186647_m1AOC377<55<5<5N.D.12N.D.
    Hs00154079_m1AOX1333110702647N.D.N.D.N.D.
    Hs00163746_m1BCHE5N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00156323_m1CBR123137424956202431
    Hs00154295_m1CBR33N.D.N.D.N.D.124N.D.N.D.342
    Hs00379036_m1CBR412035325056143421
    Hs00275607_m1CES167248101744413N.D.N.D.N.D.
    Hs00187279_m1CES23486<5<5<5<5<5<5<5
    Hs00227775_m1CES31100<5<5<5N.D.<5<5<5
    Hs00164383_m1CYP1B1191612181227559274
    Hs00221087_m1CYP20A115249287875112031
    Hs00219866_m1CYP26B112N.D.N.D.N.D.25,465N.D.N.D.N.D.
    Hs01026016_m1CYP27A138341503511<58N.D.
    Hs00168017_m1CYP27B111680739880201226465167
    Hs03044634_m1CYP2B6363<5<5<5N.D.<5712
    Hs01595322_mHCYP2C18171<5<5<5N.D.<5N.D.<5
    Hs00426380_m1CYP2C1919105429N.D.N.D.N.D.N.D.
    Hs00426397_m1CYP2C917<5912N.D.N.D.N.D.N.D.
    Hs00559370_m1CYP2E13N.D.291856N.D.77N.D.
    Hs00951113_m1CYP2J226457<5N.D.<5<537
    Hs01379776_m1CYP2R1649211660<5317278
    Hs00258076_m1CYP2S1212881039740197489
    Hs00766273_m1CYP2U136182713228113828
    Hs00214994_m1CYP2W13386316,470130630183769N.D.
    Hs00213201_m1CYP39A111N.D.N.D.N.D.108259561
    Hs00430021_m1CYP3A44N.D.N.D.N.D.N.D.N.D.196N.D.
    Hs00241417_m1CYP3A5172101716N.D.2117016
    Hs01680107_m1CYP4F114N.D.N.D.N.D.N.D.58195382
    Hs00430602_g1CYP4F12188<56N.D.N.D.<515<5
    Hs00426608_m1CYP4F256<516N.D.N.D.N.D.<5<5
    Hs01389878_m1CYP4V264151585772311
    Hs00380077_m1CYP4X13489267184N.D.N.D.N.D.N.D.
    Hs00426415_m1CYP51A132519637817819151165216
    Hs00191385_m1CYP7B114N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00949075_m1DHRS423516111647173623
    Hs00608375_m1DHRS91529<5<5<5N.D.<5<55
    Hs00559279_m1DPYD141<5<5515518N.D.
    Hs01116807_m1EPHX1434426143664611117
    Hs00157403_m1EPHX2581253518<5<5<59
    Hs00382667_m1ESD3308511710396546341
    Hs00157614_m1FMO473131311<5<56<5
    Hs00356233_m1FMO5809<5<5<5<5<58<5
    Hs00189576_m1HSD17B106689848138946383214
    Hs00186308_m1KCNAB266501815042239489
    Hs00323448_m1KDM1A130150152192144143348147
    Hs00400708_m1KDM1B39025223138262794
    Hs00165140_m1MAOA4777<5<5<514<517<5
    Hs00168533_m1MAOB941114<5N.D.272846
    Hs00168547_m1NQO113825642731547192166
    Hs00168552_m1NQO24325797252192113132178
    Hs00382210_m1PAOX285179432219286
    Hs00165563_m1PON2604779492111293658
    Hs00412993_m1PON377959656016675230399
    Hs00919949_m1PTGIS9963957075N.D.N.D.N.D.
    Hs00268403_m1SPR39856347134293647
    Hs00166578_m1SUOX11147445339174463
    Hs00233423_m1TBXAS1114N.D.N.D.N.D.27484838
    Hs00166010_m1XDH408<5<5<5N.D.<5<5N.D.
Phase II enzymes
    Hs00221125_m1AS3MT12840608948247N.D.N.D.N.D.
    Hs00241349_m1COMT92187228167309218625114
    Hs00980756_m1GGT117725263971217973
    Hs00275575_m1GSTA1632513333812<5N.D.N.D.
    Hs00155308_m1GSTA446145214137322527221
    Hs00210861_m1GSTK183021303130268825
    Hs02341469_m1GSTM17N.D.N.D.N.D.1172N.D.N.D.N.D.
    Hs00265266_g1GSTM234N.D.<5<5268N.D.N.D.N.D.
    Hs00168307_m1GSTM3515466801156N.D.N.D.160
    Hs00426432_m1GSTM421614142151<5914
    Hs00757076_m1GSTM520N.D.N.D.N.D.662N.D.N.D.N.D.
    Hs00818731_m1GSTO12583001803122289724660
    Hs00826661_m1GSTO240<5<5<5N.D.7921784
    Hs00168310_m1GSTP14243287236311121101121N.D.
    Hs00184475_m1GSTT121N.D.N.D.N.D.194271683260
    Hs01041668_m1GSTZ17628222318223423
    Hs00199373_m1HNMT69835365046112913
    Hs00198941_m1INMT595161252535<5N.D.N.D.
    Hs00220393_m1MGST1353421163058252097
    Hs00182064_m1MGST2739616153<5185127
    Hs01058946_m1MGST3257862604429<51728
    Hs00560401_m1MPST224929362523102911
    Hs00211492_m1NAA2037676841338873118134
    Hs00377717_m1NAT13189642135519N.D.46
    Hs00605099_m1NAT254<5<5N.D.N.D.N.D.N.D.N.D.
    Hs00196287_m1NNMT278994866166321N.D.
    Hs02340929_g1SULT1A2215N.D.N.D.<5N.D.N.D.<5<5
    Hs00413970_m1SULT1A3; SULT1A42549315679183543
    Hs00234899_m1SULT1B11190<5<5<5N.D.N.D.N.D.<5
    Hs00169044_m1SULT1C213102934779N.D.N.D.N.D.402
    Hs00198159_m1SULT1C435543524432050N.D.N.D.N.D.
    Hs00190268_m1SULT2B130<5N.D.N.D.N.D.6271598447
    Hs00740082_mHTPMT1355131418166550
    Hs00361812_m1TST2719<5<5<57<5166
    Hs02511055_s1UGT1A1261<5<59<5<5<57
    Hs02516990_s1UGT1A102279N.D.<5<5N.D.4631<5
    Hs01655285_s1UGT1A433<5N.D.6N.D.<5N.D.<5
    Hs01374521_s1UGT1A532N.D.N.D.<5N.D.<5N.D.5
    Hs01592477_m1UGT1A671233374816137903892993
    Hs02517015_s1UGT1A7242N.D.N.D.<5N.D.<5<5<5
    Hs01592482_m1UGT1A81043N.D.N.D.N.D.N.D.5443<5
    Hs02516855_sHUGT1A991N.D.N.D.N.D.N.D.<5N.D.<5
    Hs00226904_m1UGT2A3813<538<5<5<5<5<5
    Hs02556282_s1UGT2B10687517N.D.N.D.N.D.N.D.
    Hs01894900_gHUGT2B114579567N.D.N.D.N.D.N.D.
    Hs00854486_sHUGT2B179575<5<5<5N.D.N.D.N.D.N.D.
    Hs00426592_m1UGT2B7196818447N.D.9N.D.N.D.
    Hs00409961_m1UGT8449488150<5396230
Transporters
    Hs01059122_m1ABCA138517201245<513<5
    Hs00242232_m1ABCA243275223346220660
    Hs00975530_m1ABCA31211211267885122<5577
    Hs01105117_m1ABCA740341132277724231
    Hs00992371_m1ABCA8138N.D.N.D.N.D.272N.D.N.D.N.D.
    Hs00184491_m1ABCB11752318515N.D.N.D.23
    Hs01085315_m1ABCB102138811110775263761
    Hs00184824_m1ABCB11122N.D.N.D.N.D.N.D.N.D.N.D.<5
    Hs00240956_m1ABCB47N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00180568_m1ABCB669465717775566137305175
    Hs00188776_m1ABCB728655907748437794
    Hs00185159_m1ABCB871582971718011542
    Hs00608640_m1ABCB935344431413810955
    Hs00219905_m1ABCC16454558752485108164
    Hs00375716_m1ABCC1067122102113146496382
    Hs00358656_m1ABCC3637<516733327721
    Hs00195260_m1ABCC41111818420253110914755
    Hs00981089_m1ABCC54253715459141660
    Hs00184566_m1ABCC639851019927N.D.1516
    Hs00245340_m1ABCD49839394835208236
    Hs01053787_m1ABCG210308820171321<5
    Hs02880035_m1ABCG84N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00166067_m1AQP1111<5<5<5<58197N.D.
    Hs00798308_sHATP6V0C1571869211599397163
    Hs00163707_m1ATP7A3418382376498783
    Hs00163739_m1ATP7B11518415823439207014
    Hs00245438_m1MVP264271683294324
    Hs00166561_m1SLC10A25N.D.8222N.D.N.D.N.D.N.D.
    Hs00192639_m1SLC15A1142865784438N.D.N.D.12
    Hs00221539_m1SLC15A2151435359N.D.15N.D.
    Hs00161826_m1SLC16A1261120232717111225
    Hs00161858_m1SLC18A210N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00953345_m1SLC19A11242121172946368414987
    Hs00949693_m1SLC19A258297385360158120191140
    Hs00375596_m1SLC19A3572823273868<513<5
    Hs00188172_m1SLC1A1432725547623413615
    Hs00188193_m1SLC1A311709038969444175N.D.N.D.N.D.
    Hs00427554_m1SLC22A13446071N.D.N.D.N.D.58
    Hs00222691_m1SLC22A33N.D.N.D.N.D.N.D.30208453
    Hs00268200_m1SLC22A4205<511176425
    Hs00929869_m1SLC22A537998138<598
    Hs00185185_m1SLC25A1330015511120150475973
    Hs00188407_m1SLC28A229N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00223220_m1SLC28A3244N.D.42N.D.47342560
    Hs01085706_m1SLC29A156382432493150170415614
    Hs00155426_m1SLC29A22633539924125168259204
    Hs00983219_m1SLC29A35612614013140288627
    Hs00542001_m1SLC29A421909985698132505780115
    Hs00197884_m1SLC2A11989041045107176931195693
    Hs00977268_g1SLC31A15051079510081345244
    Hs01562168_m1SLC38A1197980599853353862
    Hs01089954_m1SLC38A25742282572049105815777
    Hs01012028_m1SLC38A5125<55<548119137306
    Hs00165789_m1SLC3A1167<5146<5<57311
    Hs00374243_m1SLC3A2336694608579351113194189
    Hs00217320_m1SLC47A120<513N.D.N.D.N.D.N.D.N.D.
    Hs00169010_m1SLC6A42519253013943N.D.N.D.17
    Hs00204928_m1SLC7A1117510732880707814711603694
    Hs00185826_m1SLC7A5174697183379913588166922173128
    Hs00938056_m1SLC7A6718514051107416146119210
    Hs00909952_m1SLC7A741170235001153733710361
    Hs00794796_m1SLC7A818815815318435<5<5166
    Hs00194554_m1SLCO2A1384<55<5<5N.D.N.D.N.D.
    Hs00200670_m1SLCO2B11941373301708<5<512
    Hs00203184_m1SLCO3A127<515<571630140
    Hs00249583_m1SLCO4A13658956369563793421009
    Hs00698884_m1SLCO4C11110327126N.D.N.D.N.D.N.D.
    Hs00229597_m1SLCO5A122N.D.N.D.N.D.N.D.275755
    Hs00388682_m1TAP1788612<55165589
    Hs00241060_m1TAP219020242314131141142
    Hs00748551_s1VDAC249636710280253743
    Hs00366592_m1VDAC333756335785121446
Nuclear receptors and transcription factors
    Hs00169233_m1AHR118953815453409990
    Hs00610222_m1AIP2811533501841165719267
    Hs01121918_m1ARNT38534504760153433
    Hs00208298_m1ARNT216N.D.N.D.N.D.21N.D.N.D.N.D.
    Hs00231733_m1CREBBP53058626554283661
    Hs00230938_m1EP30062254414341191829
    Hs00230957_m1ESR211N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00232764_m1FOXA252163123207<585189179
    Hs01054576_m1FOXO1127257132<5182715
    Hs00153153_m1HIF1A785604364185293760
    Hs00541709_m1HIF3A75441285661812N.D.N.D.N.D.
    Hs00604435_m1HNF4A2650647373<561631
    Hs00743767_sHHSP90AA1262710267134715242131
    Hs00202227_m1KEAP1299756396108456494
    Hs00186661_m1NCOA16553455542781926
    Hs00896106_m1NCOA217055514743206433
    Hs00180722_m1NCOA329211312015686274255
    Hs00196920_m1NCOR1140420212728162424
    Hs00196955_m1NCOR22129714013010156108153
    Hs00232352_m1NFE2L22150474963105243247
    Hs00222677_m1NR0B2414029042N.D.12207206414
    Hs00173195_m1NR1H271355836882812842
    Hs00172885_m1NR1H31374914449274120636
    Hs00231968_m1NR1H4115<524N.D.N.D.<514N.D.
    Hs00243666_m1NR1I2576N.D.N.D.N.D.N.D.N.D.<512
    Hs00353740_m1NR3C1122409643261N.D.N.D.N.D.
    Hs01031809_m1NR3C2431N.D.<5N.D.N.D.<5<5N.D.
    Hs00187067_m1NR5A2452217225<5<579
    Hs00231882_m1PPARA9726892537104832
    Hs00602622_m1PPARD53610171224122726
    Hs01115513_m1PPARG99818351614204131
    Hs00173304_m1PPARGC1A198N.D.N.D.N.D.N.D.<5N.D.8
    Hs00370186_m1PPARGC1B184212941<571025
    Hs00209379_m1PPRC181126127212160102101158
    Hs00832847_gHPTGES396179661167875106153
    Hs00940446_m1RARA16258824945337723
    Hs00233407_m1RARB2382238827N.D.N.D.N.D.
    Hs01559234_m1RARG50705761173333641163
    Hs01067640_m1RXRA135242704373335924
    Hs00232774_m1RXRB15554595089539945
    Hs00199455_m1RXRG13N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00268470_m1THRA28748774638268229
    Hs00230861_m1THRB1606418610197<51526
    Hs00188542_m1TRIP1132638333252151636
    Hs01045840_m1VDR165179129<5836
Other genes
    Hs00171635_m1CRABP1755377360562924N.D.N.D.N.D.
    Hs00275636_m1CRABP233798785219,011155659,109181,154527
    Hs00196206_m1GZMA134N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00188051_m1GZMB7N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00831826_s1MT1A494839356528298
    Hs00744661_sHMT1F496244913<5<5<5<5
    Hs00823168_g1MT1H6N.D.N.D.N.D.N.D.N.D.N.D.N.D.
    Hs00828387_g1MT1M280<5<5N.D.21N.D.N.D.N.D.
    Hs00745167_sHMT1X1606512<539<5<5<5
    Hs02379661_g1MT2A487812<599<5<5<5
    Hs00359394_g1MT36N.D.N.D.N.D.N.D.16349N.D.
    Hs00293639_s1MTHFR6755513533334716
    Hs00287016_m1POR59510011898534412679
    Hs00161252_m1RBP14616127<5N.D.N.D.35
    Hs00188160_m1RBP2720714790331117N.D.N.D.N.D.
    Hs00153349_m1TP53197<5<5<5515553<5
    Hs00828652_m1TXN379176476595707279
    Hs00429399_g1TXN2112143424443223124
  • TC, transverse colon; N.D., not detected.

  • a Expression value is a relative number calculated based on the assumption that the average expression level of two housekeeping genes RPLP0 and PPIA is 10,000 copies.