MRM transitions, collision energies, and declustering potentials for each P450 probe
| Metabolite | P450 | MRM | CE | DP |
|---|---|---|---|---|
| Acetaminophen | CYP1A2 | m/z 152.0 > 110.0 | 20 | 61 |
| 6α-Hydroxypaclitaxel | CYP2C8 | m/z 870.5 > 525.4 | 25 | 80 |
| 4-OH-diclofenac | CYP2C9 | m/z 312.3 > 231.1 | 30 | 60 |
| 4-OH-S-Mephenytoin | CYP2C19 | m/z 235.2 > 150.1 | 25 | 71 |
| Dextrorphan | CYP2D6 | m/z 258.1 > 199.1 | 40 | 80 |
| Desmethyl astemizole | CYP2J2 | m/z 445.1 > 121.1 | 50 | 40 |
| 1-OH-Midazolam | CYP3A4 | m/z 342.3 > 203.1 | 40 | 60 |
| Internal standard | PF-5218881 | m/z 687 > 320 | 30 | 60 |