TABLE 1

RAF values calculated from the marker activity and the contributions of AADAC and CES2 to flutamide hydrolysis in six HLH

	Hydrolase Activity		RAF		Flutamide				Contribution
	Rifampicin	CPT-11	AADAC	CES2	Concentration	Hydrolase Activity			AADAC	CES2	Total
						V_rec		V_HLH
						AADAC	CES2	V_HLH
	pmol · min⁻¹ · mg protein⁻¹				μM	pmol · min⁻¹ · mg protein⁻¹			%
rec-AADAC	41.77				5	3.1
					50	87.6
					200	389.0
rec-CES2		2.99			5		16.0
					50		38.9
					200		13.5
Liver homogenate
1	1.00	1.23	0.024	0.412	5			4.5	1.6	146.7	148.3
					50			10.6	19.7	150.7	170.4
					200			10.5	89.0	53.1	142.1
2	3.27	0.94	0.089	0.314	5			3.5	7.7	142.7	150.5
					50			16.2	48.1	75.4	123.6
					200			40.5	85.5	10.5	96.0
3	10.55	0.43	0.253	0.143	5			3.2	24.3	72.1	96.4
					50			21.2	104.2	26.3	130.5
					200			76.0	129.3	2.5	131.8
4	6.39	0.87	0.153	0.292	5			5.1	9.3	92.2	101.5
					50			28.1	47.7	40.5	88.2
					200			56.4	105.5	7.0	112.4
5	5.94	0.42	0.142	0.140	5			3.4	13.0	66.6	79.6
					50			17.9	69.5	30.3	99.8
					200			53.4	103.6	3.5	107.1
6	5.69	0.52	0.136	0.172	5			2.1	20.4	134.7	155.1
					50			14.2	83.8	47.1	130.9
					200			54.6	97.1	4.3	101.4