Identification No. | Name | Inserted (+) or Deleted (−) Restriction Enzymes | Primer Sequence (5′-3′a |
---|---|---|---|
1 | C208A | N.A. | CCCTAATCCCGCCCCAGAGTCCAG |
2 | C265A | −BsmI | GGAAGAAGGAAGCCGCCAAGACTAG |
3 | C375A | −PstI | GTCACTGCCGCCTTGCAGACCCTCG |
4 | C388A | N.A. | CTTCCACATCGCCTTCGTCAGTGG |
5 | C555A | +SpoI/NruI | CACCTGGGTCGCGACGCCCTTTCTG |
6 | C563A | +BalI/MscI | GGTGGCCTTGGCCACATTTGCCGTC |
7 | C682A | +NarI | CCAGGTGGGCGCCGGAAAGTCGTC |
8 | C730A | +EspI, SacI | ATCCTTTTTGGAGCTCAGCTGGAGG |
9 | C744A | −StuI | GATACAGGCCGCTGCCCTCCTCC |
10 | C984A | +BalI/MscI | TTCCTTTTCATGGCCAACCATGTGTCC |
11 | C1047A | +SacI/SstI | TTGGCTTCCCGAGCTCTGCACGTGG |
12 | C1105A | +PvuI | CATTGGTGCCGCGATCGTTATCCTG |
13 | C1205A | N.A. | GCGGCTGGAGGCTGTGGGCAACTG |
14 | C1209A | +PvuI | GTGTGGGCAACGCGATCGTTCTGTTTG |
15 | C1299A | +MluI | TTCCGGAACTACGCGTTGCGCTACCGAG |
16 | C1423A | +PstI | CTAGACCATGAAGCTGCAGAAGGC |
17 | C1439A | +PflMI | CCAGCTTGTGGCCCTAGCCCGGG |
18 | C1479A | N.A. | GTTCGAGGACGCCACCGTCCTCAC |
19 | Rec L | N.A. | GAAACCATCCACGACCCTAATCCCGCCCCAGAG |
20 | Rec R | N.A. | GGATTAGGGTCGTGGATGGTTTCCGAGAACAG |
21 | KbnL | N.A. | CATGGTACCATGGCGCTCCGGGGCTTCTGCAGC |
22 | KbnR | N.A. | GGCAGGATCCTTGGAGGAGTACACAACCTTC |
N.A., not applicable.
↵a Underline indicates mutated nucleotides.