MRM transitions and compound-dependent MS parameters for the detection of 6β-hydroxytestosterone, prednisolone (IS for 6β-hydroxytestosterone), 1′-hydroxymidazolam, and carbamazepine (IS for 1′-hydroxymidazolam)
| Compound | MRM Transition | Fragmentor Voltage | Collision Energy | Dwell Time |
|---|---|---|---|---|
| m/z | V | V | ms | |
| 6β-Hydroxytestosterone | 305 to 269 | 136 | 8 | 20 |
| Prednisolone (IS) | 361 to 343 | 104 | 4 | 20 |
| 1′-Hydroxymidazolam | 342 to 203 | 154 | 25 | 20 |
| Carbamazepine (IS) | 237 to 194 | 102 | 17 | 20 |