TABLE 2

Summary of LC-MS/MS conditions for repaglinide metabolites investigated and internal standard with details on mass transitions and retention times

CompoundInternal StandardElectrospray IonizationTransitionRetention Time
min
2-Despiperidyl-2-amino repaglinide (M1)IndomethacinPositive385.35 > 162.152.7
2-Despiperidyl-2-(5-carboxypentylamine) repaglinide (M2)IndomethacinPositive485.5 > 194.22.9
3′-Hydroxy repaglinide (M4)IndomethacinPositive469.45 > 246.32.8
Repaglinide acyl-β-d-glucuronideIndomethacinPositive629.4 > 230.43.4
IndomethacinN.A.Positive358.1 > 139.13.0
  • N.A., not applicable.