Biochemical/biophysical characterization of the humanized mAbs
Tm, Tm1, Tm2, and Tm Fab show the Tm values for the CH2, CH3, and Fab regions of each mAb, respectively.
Heparin binding, data show percent of IgG that remains bound to heparin relative to background well containing no antibody.
| mAb | Tm | pI | Heparin Binding | ||
|---|---|---|---|---|---|
| Tm1 | Tm2 | Tm Fab | |||
| °C | |||||
| A1 WT | 67.7 | 71.4 | 75.1 | 7.75 | 20 |
| A1 V308P | 60.4 | 70.6 | 74.8 | 7.69 | 22 |
| A1 T250Q/M428L | 63.2 | 70.8 | 75.0 | 7.70 | 49 |
| B1 WT | 69.8 | 70.0 | 81.3 | 7.58 | 3 |
| B1 V308P | 61.5 | 73.9 | 81.3 | 7.61 | 0 |
| B1 T250Q/M428L | 65.4 | 71.2 | 81.2 | 7.64 | 5 |
| C1 WT | 66.8 | 69.3 | 71.8 | 7.41 | 0 |
| C1 V308P | 60.3 | 68.6 | 71.4 | 7.48 | 13 |
| C1 T250Q/M428L | 62.4 | 67.7 | 71.3 | 7.54 | 4 |
| D1 WT | 64.7 | 66.8 | 68.8 | 7.57 | 35 |
| D1 V308P | N.A. | N.A. | N.A. | N.A. | 0 |
| D1 T250Q/M428L | 61.7 | 64.5 | 68.0 | 7.57 | 63 |
| E1 WT | 69.7 | 74.0 | 78.3 | 6.64 | 0 |
| E1 V308P | 61.8 | 73.0 | 78.1 | 6.56 | 4 |
| E1 T250Q/M428L | 64.9 | 73.7 | 78.0 | 6.64 | 0 |
N.A., data not available.