TABLE 1

Mass spectrometer settings and LC conditions used in the analysis of parent compounds and metabolites

Parent/MetaboliteBiotransformation Reaction(s)LC ConditionsQ1Q3CE
NaproxenLC1229169−40
    Naproxen acyl glucuronideGlucuronidation405229, 185, 169a−40
CarbamazepineLC1237194,a 11727, 43
    Carbamazepine-10,11-epoxideOxidation253210,a 18027, 43
Salicylic acidLC2137109, 93a−26
    2,3-Dihydroxybenzoic acidOxidation153109†−26
SulfadiazineLC1251156, 108a27, 35
    N-Acetyl sulfadiazineN-Acetylation293185, 134,a 10827, 35, 35
MethylphenidateLC1234128,a 11565, 69
    Ritalinic acidHydrolysis220128,a 11565, 69
Nicotine Δ1′,5′-iminium ionLC3161130,a 11835
    CotinineOxidation177118, 98, 80a35

  • CE, collision energy.

  • ↵a Daughter ions were used in MRM transitions for peak area ratio measurements.