TABLE 2

Sequence alignment of the candidate peptide of human UGT1A9 as an antigen with highly similar rodent Ugt1a isoforms

Multiple sequence alignment was performed using the ClustalW2 program (http://www.ebi.ac.uk/Tools/msa/clustalw2/) for amino acid residues 87 to 118 and those of the corresponding region of rodent Ugt1a isoforms. Amino acids that are identical with those of UGT1A9 are in bold type.

IsoformAccession No.SequenceIdentity with UGT1A9 (%)
87–118Full-Length
Human UGT1A9NP_06630787DREFKAFAHAQWKAQVRS-IYSLLMGSYNDIFD118
Mouse Ugt1a7cAAP4859887NREFKISIDAQWKSQQEGGILPLLDSPAKGFFE1194077
Mouse Ugt1a9AAP4859985DREFKYLSYTQWKTPEHS-IRSFLTGSARGFFE1164778
Mouse Ugt1a10AAP4860087DREFKYFTYTQWKTPEQS-IRSFMTGSARGFFE1184778
Rat Ugt1a7AAR9563587NREFKFFIDSQWKTQQEGGVLPLLTSPAQGFFE1193677
Rat Ugt1a8AAR9563687NYHFKFFAHNQWKTQEVG-MFSLLKHSGKGFFE1184479
Rat Ugt1a10AAR9563087DREFKHFSYTQWKTPEQS-MYSLITGSVKDFLE1185076