TABLE 1

Masses from the deconvoluted spectra of CYP2E1, cytochrome P450 reductase, and cytochrome b5 isolated from incubations containing AMAP, NADPH, or a combination thereof

ComponentDetected Massesn
CYP2E1ReductaseCytochrome b5K+ Heme
+NADPH, +AMAP54,622.4 ± 8.9 54,791.3 ± 6.177,712.2 ± 2.415,949.3 ± 0.6657.3 ± 0.16
54,791.3 ± 6.1
+AMAP54,458.7 ± 3.377,719.2 ± 5.115,949.0 ± 1.4657.3 ± 0.05
+NADPH54,465.5 ± 2.077,721.3 ± 1.315,949.0 ± 1.2657.3 ± 0.22
CYP2E1 standard54,451.7 ± 5.5N.A.N.A.657.0 ± 0.33
P450 reductase standardN.A.77,714.5 ± 0.7N.A.N.A.2
Cytochrome b5 standardN.A.N.A.15,948.8 ± 0.4657.3 ± 0.02
  • N.A., not applicable.