Comparison of clearance and Km values obtained by the conventional and media loss methods
| Compound | Conventional Methoda | Media-Loss Methoda | Ratio of Clearance by Two Methods | ||
|---|---|---|---|---|---|
| CLint | Km | CLint | Km | ||
| μl · min−1 · 106 cells−1 | μM | μl · min−1 · 106 cells−1 | μM | ||
| Atorvastatin | 113 ± 38 | 1.3 ± 0.7 | 459 ± 233 | 0.43 ± 0.24 | 4.1 |
| Clarithromycin | 46 ± 11 | 68 ± 11 | 1.5 | ||
| Erythromycin | 26 ± 15 | 3.5 ± 0.9 | 35 ± 20 | 5.5 ± 3.5 | 1.2 |
| Fexofenadine | 17 ± 1.7 | 4.7 ± 2.1 | 117 ± 16 | 7.0 | |
| Pitavastatin | 7.1 ± 2.3 | 1705 ± 466 | 13 | 240 | |
| Repaglinide | 49.9 ± 3.5 | 49.3 ± 14 | 1.0 | ||
| Rosuvastatin | 3.3 ± 1.9 | 1657 ± 849 | 498 | ||
| Saquinavir | 1675 ± 69 | 2.1 ± 1.2 | 1469 ± 208 | 1.39 | 0.9 |
↵a Mean ± S.D. for n = 3.