CYP2E1-mediated chlorzoxazone hydroxylase activity after a 72-min preincubation
Values are the average of two experiments conducted in triplicate on separate days (control activity = 16.5 ± 0.6 pmol · min−1 · pmol−1).
| Preincubation Components | %Control Activity |
|---|---|
| CYP2E1 Supersomes | 100 |
| +NADPH | 88.5 ± 2.6 |
| +2.5 mM AMAP | 97.6 ± 2.5 |
| +20 mM AMAP | 89.3 ± 2.8 |
| +40 mM AMAP | 65.7 ± 2.6 |
| +20 mM AMAP, +NADPH | 64.8 ± 6.1 |
| +20 mM AMAP, +NADPH, +5 mM glutathione | 91.3 ± 2.8 |
| +40 mM AMAP, +NADPH | 43.7 ± 3.4 |
| +0.25 mM 1-aminobenzotriazole | <1 |