TABLE 1

Kinetic constants for E2 and EE2 sulfation determined with pure SULT1A1

Reactions were performed as described under Materials and Methods with four different concentrations of E2 or EE2 and PAPS as denoted under Materials and Methods.

KmVmaxVmax/Km
SubstratePAPS
μMpmol · min−1 · μg−1
E22.4 ± 0.90.36 ± 0.0616 ± 2.16.7
EE20.69 ± 0.130.45 ± 0.1211 ± 0.7715.9