Main kinetic parameters for the formation of S-6,7-diG in incubations of S-6-G and S-7-G with microsomes and human recombinant UGT, respectively
| Incubation of S-7-G | Incubation of S-6-G | |||||||
|---|---|---|---|---|---|---|---|---|
| Km | Vmax | Ki | Vmax/Km | Km | Vmax | Ki | Vmax/Km | |
| μM | nmol · min−1 · mg protein−1 | μM | μl · min−1 · mg protein−1 | μM | nmol · min−1 · mg protein−1 | μM | μl · min−1 · mg protein−1 | |
| HIM | 643 ± 69 | 11.9 ± 1.2 | 59.5 ± 6.9 | 18.5 | 156 ± 98 | 17.1 ± 9.7 | 11.8 ± 7.4 | 110 |
| HLM | 62.4 ± 10.0 | 0.736 ± 0.084 | 133 ± 28 | 11.8 | 123 ± 44 | 15.6 ± 4.8 | 24.0 ± 8.8 | 127 |
| RIM | 349 ± 125 | 6.60 ± 2.07 | 79.0 ± 31.2 | 18.9 | 41.1 ± 9.9 | 1.23 ± 0.20 | 79.2 ± 20.0 | 30.0 |
| RLM | 15.2 ± 2.3 | 3.10 ± 0.23 | 302 ± 75 | 204 | 21.2 ± 1.5 | 1.94 ± 0.08 | 78.3 ± 5.9 | 91.3 |
| UGT1A1 | 174 ± 52 | 1.94 ± 0.47 | 96.6 ± 32.1 | 11.2 | ||||
| UGT1A8 | 198 ± 21 | 0.319 ± 0.017 | 1.61 | 58.3 ± 6.0 | 0.465 ± 0.017 | 7.96 | ||
| UGT1A10 | 225 ± 105 | 0.903 ± 0.337 | 208 ± 126 | 4.01 | 78.2 ± 70.2 | 0.221 ± 0.133 | 242 ± 277 | 2.84 |