Activator | Km | Vmax | Vmax/Km |
---|---|---|---|
μM | nmol · min−1 · mg protein−1 | ||
Kinetics by varying 4-MUa | |||
Brij-58 | 93.6 ± 0.3 | 26.3 ± 0.3 | 0.28 |
Alamethicin | 163 ± 9 | 8.32 ± 0.15 | 0.05 |
Kinetics by varying UDP-GlcUAb | |||
Brij-58 | 348 ± 22 | 19.0 ± 0.3 | 0.05 |
Alamethicin | 586 ± 49 | 4.73 ± 0.11 | 0.01 |
↵a 4-MU glucuronide formation was estimated at 10 concentrations of 4-MU (10–5000 μM). The concentration of UDP-GlcUA was set at 2 mM in this assay.
↵b 4-MU glucuronide formation was estimated at 10 concentrations of UDP-GlcUA (50–7500 μM). The concentration of 4-MU was set at 100 μM in this assay. Each value represents the optimized value ± S.E.