Previous studies that described the enzyme kinetic mechanism of UGT enzymes
| Enzyme Source | Aglycone Substrate | Reaction Mechanism | Reference |
|---|---|---|---|
| Guinea pig liver microsomes | Bilirubin | Compulsory-order ternary complex (UDPGA first, aglycone second) or iso-Theorell-Chance | Potrepka and Spratt, 1972 |
| Beef and guinea pig liver microsomes | p-Nitrophenol | Rapid equilibrium random-order ternary complex | Vessey and Zakim, 1972 |
| Rat liver microsomes | Morphine | Compulsory-order ternary complex (UDPGA first, aglycone second) | Sanchez and Tephly, 1975 |
| Pig kidney microsomes | Estrone | Ternary-complex mechanism, iso-Theorell-Chance (aglycone first, UDPGA second) | Rao et al., 1976 |
| Purified UGT from rat liver | Chenodeoxycholic acid and testosterone | Ternary-complex mechanism | Matern et al., 1982 |
| Rat intestinal microsomes | 1-Naphthol | Compulsory-order ternary complex (aglycone first, UDPGA second) | Koster and Noordhoek, 1983 |
| Two purified UGTs from rat liver | Androsterone and testosterone | Rapid equilibrium random-order ternary complex | Falany et al., 1987 |
| Purified UGT from human liver | Hyodeoxycholic acid | Ternary-complex mechanism | Matern et al., 1991 |
| Purified UGT from rat liver microsomes | Substituted phenols | Random-order ternary complex | Yin et al., 1994 |
| Recombinant human UGTs from 1A family | Entacapone, scopoletin, umbelliferone, 1-naphthol, 4-hydroxyestrone, ethinylestradiol | Compulsory-order ternary complex (UDPGA first, aglycone second) | Luukkanen et al., 2005 |
| Recombinant human UGT1A6 | Scopoletin | Compulsory-order ternary complex (UDPGA first, aglycone second) | Patana et al., 2007 |