Relative mRNA expression of organic anion transporters in Caco-2 cells
Data are expressed as mean ± S.D. (n = 3).
| Common Name | Gene Symbol | GenBank Accession Number | Primer Sequence | Amplicon Length | Relative mRNA Expression (per Million β-Actin) | |
|---|---|---|---|---|---|---|
| Forward | Reverse | |||||
| bp | ||||||
| OATP1A2 | SLCO1A2 | NM_134431 | 5′-ggggcatgcaggatatatga-3′ | 5′-tggaacaaagcttgatcctctta-3′ | 77 | 101 ± 6 |
| OATP1B1 | SLCO1B1 | NM_006446 | 5′-ttcaagtggcaataaaaagccta-3′ | 5′-cacccaaatgggctgagtaa-3′ | 95 | Undetectable |
| OATP1B3 | SLCO1B3 | NM_019844 | 5′-ccatcactcaaatagaaaggagatt-3′ | 5′-acaatcacaagcaaatttccaa-3′ | 94 | Undetectable |
| OATP2B1 | SLCO2B1 | NM_007256 | 5′-ggcgaaaggtcttagcagtc-3′ | 5′-gcttagagggagagtccttgc-3′ | 63 | 2702 ± 157 |
| OSTα | OSTalpha | NM_152672 | 5′-ctgaagaccaattacggcatc-3′ | 5′-gagggcaagttccacagg-3′ | 96 | 529 ± 89 |
| OSTβ | OSTbeta | NM_178859 | 5′-gctgtggtggtcattataagcat-3′ | 5′-gtggctgcatcgtttctttt-3′ | 76 | 5885 ± 299 |
| β-Actin | ACTB | NM_001101 | 5′-attggcaatgagcggttc-3′ | 5′-ggatgccacaggactccat-3′ | 95 | Control |