Method validation of optimized HPLC systems
| Flavonoids | Precision (%RSD) | LOD | LOQ | Linearity | Accuracy(% Error) | |||
|---|---|---|---|---|---|---|---|---|
| Conc. | Intraday | Interday | 1 | 50 | ||||
| μg/ml | μg/ml | R2 | μg/ml | |||||
| 2 | 3.56 | 5.99 | ||||||
| DMF | 5 | 1.31 | 5.51 | 0.93 | 1.86 | y = 9.027x + 3.646 (R2 = 0.996) | 6.12 | 0.43 |
| 10 | 2.05 | 2.60 | ||||||
| 20 | 1.23 | 4.18 | ||||||
| 40 | 0.53 | 2.29 | ||||||
| PMF | 2 | 3.85 | 5.00 | 0.19 | 0.37 | y = 20.771x + 11.987 (R2 = 0.993) | 3.23 | 2.16 |
| 5 | 0.86 | 3.66 | ||||||
| 10 | 1.39 | 6.38 | ||||||
| 20 | 1.40 | 7.47 | ||||||
| 40 | 0.32 | 3.55 | ||||||
| TMF | 2 | 4.89 | 5.00 | 0.09 | 0.19 | y = 74.471x + 56.212 (R2 = 0.993) | 2.80 | 2.49 |
| 5 | 1.04 | 6.38 | ||||||
| 10 | 0.71 | 7.31 | ||||||
| 20 | 0.32 | 7.73 | ||||||
| 40 | 0.39 | 4.07 | ||||||
Conc., concentration: RSD, relative S.D. (calculated from S.D. divided by mean and multiplied by 100).