Formation of M3-1 to M3-4 and 11-hydroxy-NBP catalyzed by recombinant CYP enzymes
| P450 | M3-1a | M3-2 | M3-3 | M3-4 | 11-Hydroxy-NBP |
|---|---|---|---|---|---|
| % | |||||
| 1A2 | n.d. | 5.6 | 32 | 15 | 26 |
| 2A6 | 11 | 3.4 | 28 | 43 | 12 |
| 2B6 | 7.6 | 28 | n.d. | n.d. | 25 |
| 2C8 | 2.7 | 1.1 | n.d. | n.d. | 2.6 |
| 2C9 | 4.8 | 2.0 | n.d. | n.d. | 6.7 |
| 2C19 | 0.9 | 27 | n.d. | n.d. | 3.6 |
| 2D6 | 0.5 | 1.3 | 2.0 | n.d. | 9.8 |
| 2E1 | 30 | 31 | 37 | 42 | 13 |
| 3A4 | 40 | n.d. | n.d. | n.d. | n.d. |
| 3A5 | 20 | n.d. | n.d. | n.d. | n.d. |
| 4A11 | n.d | n.d. | n.d | n.d. | n.d. |
n.d, Not detected.
aThe chromatographic peak area of the metabolites detected in individual recombinant CYP450 isoform was normalized with respect to the nominal specific content of the corresponding CYP in native HLMs. Each CYP450 isoform is expressed as the percentage of the total normalized peak area.