Primer sequences used for quantitation of human constitutive androstane receptor (CAR) splicing variants (SVs) in Fig. 1
| Assay ID | Location | Primer Sequence (5′ to 3′) |
|---|---|---|
| WT-SV5-SV8 | Forward | CCAGGTAGAGTTTTTGGAGTTGCT |
| Reverse | GCAGCCAAGAGCACATACTCA | |
| Reporter | FAM-CTTGGAGCTGCAGTTTT | |
| SV2 | Forward | TGCCACCCAACTTGTCCTT |
| Reverse | GCAGCTGATCAATCTCATCTCTCT | |
| Reporter | FAM-CAGGTCGGTCTGTAAGATA | |
| SV4-SV7 | Forward | GCTCCAAGAGCCTGAGTATGTG |
| Reverse | TCCAGGTCGGTCTGTAAGATAGG | |
| Reporter | FAM-CCATGGCCCTCTTCTC | |
| SV9 | Forward | GTGACCGACCTGGAGTTACC |
| Reverse | TTGCAGAGTCAGTGCCATCTC | |
| Reporter | FAM-CTTGCAGCTGATCAATC | |
| SV10 | Forward | GCTCCAAGAGCCTGAGTATGTG |
| Reverse | TCCAGGTCGGTCTGTAAGATAGG | |
| Reporter | FAM-CCATGGCCCTCTTCTC |