Effects (percentage of control activity) of various concentrations of SMI, isolated PXT, and standard PXT on 4-hydroxylation and 1′-hydroxylation of MDZ in RLM, HLM, and recombinant CYP3A4 (rCYP3A4) systems compared with the corresponding control groups
| Groups | 4-OH MDZ | 1′-OH MDZ | ||||
|---|---|---|---|---|---|---|
| RLM | HLM | rCYP3A | RLM | HLM | rCYP3A | |
| Control (%) | 100 | 100 | 100 | 100 | 100 | 100 |
| 10% SMI | 34.8 | 36.9 | 53.4 | 199.3 | 186.8 | 184.6 |
| 2.0 μg/ml isolated PXT | 45.3 | 39.2 | 50.6 | 244.6 | 181.8 | 198.3 |
| 2.0 μg/ml standard PXT | 42.4 | 39.3 | 48.7 | 226.9 | 196.7 | 203.4 |
| 5% SMI | 51.1 | 54.8 | 59.5 | 150.8 | 158.9 | 162.3 |
| 1.0 μg/ml isolated PXT | 64.2 | 58.8 | 68.0 | 202.1 | 168.1 | 151.8 |
| 1.0 μg/ml standard PXT | 62.5 | 57.5 | 66.8 | 204.6 | 169.9 | 155.5 |
| 2.5% SMI | 65.9 | 64.5 | 68.1 | 133.5 | 141.0 | 142.0 |
| 0.5 μg/ml isolated PXT | 74.4 | 70.4 | 75.2 | 132.2 | 135.9 | 137.9 |
| 0.5 μg/ml standard PXT | 70.5 | 71.2 | 73.7 | 137.9 | 145.3 | 144.8 |
HLM, human liver microsome; MDZ, midazolam; RLM, rat liver microsome; PXT, panaxytriol; SMI, Shenmai injection.