PAP Binding | Untreated | Pretreatment | |||
---|---|---|---|---|---|
1 mM GSH | 1 mM GSSG | 0.2 mM Diamide | 0.5 mM DTNB | ||
No DHEA | |||||
Kd1 (µM) | 1.9 ± 0.2 | 0.9 ± 0.3a | 2.4 ± 0.3 | 2.0 ± 0.3 | 3.1 ± 1.1 |
Kd2 (µM) | 540 ± 100 | 661 ± 194 | 250 ± 38a | 340 ± 54 | 325 ± 52 |
0.5 µM DHEA | |||||
Kd1 (µM) | 0.9 ± 0.2 | 1.6 ± 0.2a | 0.8 ± 0.1 | 1.2 ± 0.3 | 9.4 ± 3.2a |
Kd2 (µM) | 248 ± 92 | 328 ± 94 | 484 ± 70 | 236 ± 50 | >1000a |
50 µM DHEA | |||||
Kd1 (µM) | 1.1 ± 0.3 | 0.6 ± 0.1 | 44 ± 27 | 1.7 ± 0.3 | 0.8 ± 0.1 |
Kd2 (µM) | 264 ± 36 | 453 ± 63a | >1000a | 421 ± 85 | 257 ± 31 |
↵a Kd value was different (P < 0.05) from the corresponding dissociation constant for untreated hSULT2A1.