TABLE 1

Kinetic parameters for OM-hydrolase activities by wild-type and mutant proteins of human CMBL

Data generated in triplicate determinations were fitted to the single enzyme Michaelis-Menten model by nonlinear least-squares regression.

Recombinant CMBLKmVmaxVmax/Km
μMnmol/min/mg proteinml/min/mg protein
Wild-type119351029.5
Y155C156204013.1
A150T114403035.4