TABLE 2

Hematologic and morphologic differences in SAGE knockout rats

Rats	Mdr1a Knockout	Bcrp Knockout	Mrp2 Knockout
Number of animals/group	4 males	4 males	4 males
	Percent change from wild type
Body weight compared with wild type	↑18*	↑17*	↑32*
Hematology	Percent change from wild type^{^a}, severity^{^b}
Reticulocyte count	—	↓25, MI	↓36, SL
Hemoglobin	↓8, EQ	—	—
Neutrophil count	—	↓31, MI	↓51, SL
Clinical chemistry	Percent change from wild type, severity
Urea nitrogen	↓10, EQ	↓16, EQ	↓39, SL
Creatinine	↓12, EQ	↓ 8, EQ	↓37, SL
Total bilirubin	—^{^c}	–^{^c}	↑3800, MA
ALP	↑72, MI	↑65, MI	—
ALT	↓14, EQ	↓ 21, EQ	↓ 24, EQ
AST	↓29, MI	↓9, EQ	↓ 18, EQ
CK	↓32, EQ	—	↓51, EQ
Glucose	↑26, EQ	↑39, MI	↑50, MI
Cholesterol	↓23, MI	↓32, SL	↑25, EQ
Triglyceride	↑89, SL	↓32, SL	↑62, MI
Relative organ weights^{^d}	Percent change from wild type, severity
Kidney	↑24*, SL	↑30*, SL	↑44*, MO
Liver	↑28*,MI	↑22*, MI	↑53*, MO
Morphologic pathology	Number of animals, severity
Liver
Enlarged (gross)	—	—	1 MO
Increased hepatocyte size	3 MI-SL	—	4 MI-SL
Kidney
Discoloration, green (gross)	—	—	4 MI-SL
Increased glomerular size	—	4 MI	3 MI
Increased hyaline droplets, PCT	—	—	4 SL
Basophilic tubule	2 MI	1 MI	3 MI-SL
Cerebrum
Ventricular dilation	—	3 SL	—
Cerebellum
Molecular layer aplasia, focal	1 P	—	—

—, not different from wild type or finding not present; ↑, increased; ↓, decreased; ALP, alkaline phosphatase; ALT, alanine aminotransferase; AST, aspartate transaminase; CK, creatine kinase.
↵^a Percent change from wild type = [(knockout value – wild type value)/(wild-type value)] × 100%.
↵^b Severity grading scale: present, no grade assigned (P), equivocal (EQ), minimal (MI), slight (SL), moderate (MO), marked (MA), severe (SE).
↵^c All individual total bilirubin values for this group were below the lower limit of quantification for the assay.
↵^d Organ weight relative to body weight.
^* P < 0.05