TABLE 8

Substrate uptake in vesicles in presence and absence of ATP

LaboratoryVesicle sourceProbe SubstrateSubstrate ConcentrationATP-Dependent Uptake RateATP-Dependent Uptake ClearanceUptake Ratio +ATP/–ATP
µMpmol/min 
per milligramµl/min 
per milligram
20Solvo3H-N-Methylquinidine2514 ± 104257 ± 529.4
21Solvo3H-N-Methylquinidine152 ± 3152 ± 3123.9
22SolvoN-Methylquinidine26.7 ± 1.93.3 ± 0.935.9
23Internal3H-N-Methylquinidine1226 ± 58226 ± 5815.6
26-1Internal3H-Vinblastine0.14.3 ± 2.043 ± 204.7
26-2Solvo3H-Xinblastine0.12.9 ± 1.229 ± 125.7