Cell Line Data Only | All Systems | ||||||||
---|---|---|---|---|---|---|---|---|---|
Response | A>B w/o Inhibitor | B>A w/o Inhibitor | Log ER1 w/o Inhibitor | A>B with Inhibitor | B>A with Inhibitor | Log ERa with Inhibitor | Log IC50 A>Ba | Log IC50 B>Aa | Log IC50 B>A or Uptake into Inside-Out Vesiclea |
Cells/systems | 9 | 0 | 8 | 7 | 8 | 0 | 13 | 14 | 28 |
Labs | 77 | 82 | 62 | 78 | 79 | 45 | 35 | 35 | 29 |
Cell/system by experimentb | 0 | 0 | 0 | 0 | 0 | 0 | Not modeled | Not modeled | Not modeled |
Lab by experimentc | 9 | 14 | 20 | 6 | 8 | 29 | Not modeled | Not modeled | Not modeled |
Error | 5 | 4 | 10 | 8 | 5 | 25 | 52d | 51 d | 43 d |
Total | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
↵a Log ER and log IC50 were used in variance component analysis since data are log normally distributed. ER, efflux ratio.
↵b Interaction between experiment and cell/system (for each time the experiment was performed, this is the variability in probe substrate transport that can be ascribed to differences between the three cell lines/four experimental systems).
↵c Interaction between experiment and laboratory (for each time the experiment was performed, this is the variability in probe substrate transport that can be ascribed to differences between the laboratories).
↵d Variability due to replication, which in this case constitutes interactions between experiment and cell/system, experiment and laboratory, well-to-well variability and measurement variability that could not be further deconvoluted because only a single IC50 value for each inhibitor was used. While the vesicle laboratories generated two sets of IC50 values for most compounds, only day 1 vesicle-IC50 values were included for consistency with the cell-based systems.