Aspirin hydrolysis by HSA and plasma of human and several animals and the effect of esterase inhibitors EDTA, BNPP, and ethopropazine on their hydrolase activities
Control refers to hydrolysis conditions in the absence of inhibitors at a constant concentration of aspirin (2.5 mM). Data show statistically significant differences from control activity in the absence of inhibitors. Values represent the mean ± S.D. (n = 3). Values in parentheses represent the remaining hydrolase activities (%) after inhibition by the relevant inhibitor.
| Species | Hydrolase Activity in Controls | Hydrolase Activity in the Presence of Esterase Inhibitors | |||
|---|---|---|---|---|---|
| 1 mM EDTA | 0.5 mM BNPP | 0.5 mM Ethopropazine | 0.5 mM Ethopropazine and 0.5 mM BNPP | ||
| nmol/min per milliliter plasma | |||||
| HSA (4%) | 4.82 ± 0.15 | 3.96 ± 0.46 (82.1 ± 9.47) | 4.04 ± 0.40 (83.9 ± 9.78) | 5.51 ± 0.58 (113 ± 14.8) | |
| Human | 21.6 ± 0.81 | 10.7 ± 0.63 (49.5 ± 5.50)*** | 15.1 ± 2.43 (70.3 ± 19.8)* | ||
| Monkey | 19.5 ± 2.14 | 12.3 ± 1.60 (63.2 ± 5.54)* | 15.3 ± 5.04 (76.6 ± 37.6) | ||
| Dog | 7.51 ± 1.09 | 5.13 ± 1.46 (68.3 ± 8.67)* | 7.06 ± 1.59 (93.7 ± 12.6) | ||
| Minipig | 4.79 ± 1.66 | 4.83 ± 0.20 (101 ± 3.12) | 3.74 ± 1.67 (78.2 ± 9.21) | ||
| Rabbit | 37.0 ± 2.58 | 25.4 ± 4.60 (68.6 ± 11.6)* | 3.78 ± 0.26 (10.2 ± 0.24)** | 32.7 ± 13.5 (88.3 ± 7.31) | |
| Mouse | 12.3 ± 4.68 | 12.0 ± 0.37 (98.6 ± 15.6) | 1.18 ± 0.35 (10.0 ± 3.50)* | 20.5 ± 1.74 (173 ± 54.6)* | 0.76 ± 0.61 (6.75 ± 5.47)* |
| Rat | 15.5 ± 2.81 | 16.0 ± 2.03 (103 ± 4.68) | 2.27 ± 0.97 (14.7 ± 4.72)* | 71.8 ± 14.3 (468 ± 70.9)** | 3.65 ± 0.45 (26.4 ± 5.77)* |
↵* P < 0.05; **P < 0.01; ***P < 0.001.