TABLE 1

Identifications of degarelix and metabolites of degarelix with use of product ion mass spectrometry data

Analyte	Precursor Ion z	Characteristic Product Ions
	m/z	m/z
Degarelix	816.4	121.1, 154.1, 170.1, 171.2, 186.1 (y₂”), 240.1 (b₁), 356.3 (y₃”), 421.2 (b₂), 469.4 (y₄”), 569.2 (b₃), 606.3 (y₈”), 656.1 (b₄), 674.4 (y₅”), 696.9 (y₉”), 794.9 (y₁₀”), 807.9 (b₁₀), 958.4 (b₅), 976.6 1 (y₆”), 1063.6 1 (y₇”), 1163.6 (b₆), 1211.7 (y₈”), 1276.5 (b₇), 1393.0 (y₉”)
FE 200486(1-10)-OH	816.9	121.1, 154.1, 170.1, 171.2, 187.1 (y₂”), 240.1 (b₁), 357.3 (y₃”), 421.2 (b₂), 470.4 (y₄”), 569.2 (b₃), 606.8 (y₈”), 656.1 (b₄), 675.4 (y₅”), 697.4 (y₉”), 795.4 (y₁₀”), 807.9 (b₁₀), 958.4 (b₅), 977.6 1 (y₆”), 1064.6 1 (y₇”), 1163.6 (b₆), 1212.7 (y₈”), 1276.5 (b₇)
FE 200486(1-9)-OH	781.4	121.1, 154.1, 170.1, 171.2, 240.1 (b₁), 286.3 (y₂”), 399.4 (y₃”), 421.2 (b₂), 569.2 (b₃), 604.6 (y₄”), 656.1 (b₄), 662.0 (y₈”), 760.1 (y₉”), 773.6 (b₉), 906.8 (y₅”), 958.4 (b₅), 993.8 (y₆”), 1141.9 (y₇”), 1163.6 (b₆), 1323.2 (y₈”), 1276.5 (b₇)
FE 200486(1-7)-OH	1294.5	121.1, 154.1, 240.1 (b₁), 337.2 (y₂”), 421.2 (b₂), 569.2 (b₃), 639.3 (y₃”), 656.1 (b₄), 726.3 (y₄”), 874.4 (y₅”), 958.4 (b₅), 1055.4 (y₆”), 1163.6 (b₆), 1251.5 (y₇”), 1276.5 (b₇)
FE 200486(1-6)-OH	1181.4	121.1, 154.1, 240.1 (b₁), 421.2 (b₂), 526.3 (y₂”), 569.2 (b₃), 613.2 (y₃”), 656.1 (b₄), 761.3 (y₄”), 942.3 (y₅”), 958.4 (b₅), 1139.4 (y₆”), 1163.6 (b₆)
FE 200486(1-5)-OH	976.4	121.1, 154.1, 240.1 (b₁), 321.2 (y₁”), 408.1 (y₂”), 421.2 (b₂), 556.2 (y₃”), 569.2 (b₃), 656.1 (b₄), 737.4 (y₄”), 934.3 (y₅”), 958.4 (b₅)
FE 200486(1-4)-OH	674.3	121.1, 154.1, 240.1 (b₁), 254.1 (y₂”), 421.2 (b₂), 435.1 (y₃”), 569.2 (b₃), 632.2 (y₄”), 656.1 (b₄)
Oxidation in D-2NaI amino acid	824.5	154.1, 171.2, 186.1 (y₂”), 256.1 (b₁), 356.3 (y₃”), 437.2 (b₂), 469.4 (y₄”), 585.2 (b₃), 606.3 (y₈”), 672.1 (b₄), 674.4 (y₅”), 696.9 (y₉”), 802.9 (y₁₀”), 815.9 (b₁₀), 974.4 (b₅), 976.6 1 (y₆”), 1063.6 1 (y₇”), 1179.5 (b₆), 1211.7 (y₈”), 1292.7 (b₇), 1393.0 (y₉”)
Oxidation in D-3Pal amino acid	824.5	154.1, 171.2, 186.1 (y₂”), 240.1 (b₁), 356.3 (y₃”), 421.2 (b₂), 469.4 (y₄”), 585.2 (b₃), 614.3 (y₈”), 672.1 (b₄), 674.4 (y₅”), 704.9 (y₉”), 802.9 (y₁₀”), 815.9 (b₁₀), 974.4 (b₅),976.6 1 (y₆”), 1063.6 1 (y₇”), 1179.6 (b₆), 1227.6 (y₈”), 1292.6 (b₇), 1409.0 (y₉”)