Primers used for real-time quantitative PCR
| S. No. | mRNA | Sequence (5′ → 3′) |
|---|---|---|
| 1 | GAPDH-FP | GGACCACCAGCCCCAGCAAGAG |
| 2 | GAPDH-RP | GAGGAGGGGAGATTCAGTGTGGTG |
| 3 | AKR1D1-FP | TCAGAACCTAAATCGACCCCT |
| 4 | AKR1D1-RP | CCCTGTGTCAATAGCAACCTTC |
| 5 | CYP3A4-FP | CCTTACACATACACACCCTTTGGAAGT |
| 6 | CYP3A4-RP | AGCTCAATGCATGTACAGAATCCCCGGTTA |
| 7 | CYP2C8-FP | GCAGGAAAAGGACAACCAAA |
| 8 | CYP2C8-RP | GTGTAAGGCATGTGGCTCCT |
| 9 | CYP2C9-FP | GATCTGCAATAATTTTTCTC |
| 10 | CYP2C9-RP | TCTCAGGGTTGTGCTTGTC |
| 11 | CYP2C19-FP | GAGGAGTTTTCTGGAAGAGGCC |
| 12 | CYP2C19-RP | CATTGCTGAAAACGATTCCAAA |
| 13 | CYP2B6-FP | CACCCTAACACCCATGACCG |
| 14 | CYP2B6-RP | GATCACACCATATCCCCGGA |
| 15 | FXR-FP | TATCTAGCCCAATATTTACAGTT |
| 16 | FXR-RP | GTATAACTTTCCTTTATTTCAC |
| 17 | CYP7A1-FP | AAACTGGATGGAAATAGCCATTTG |
| 18 | CYP7A1-RP | TTTCATCTGCAAGTCATTTAGCG |
| 19 | SHP-FP | GCTGTCTGGAGTCCTTCTGG |
| 20 | SHP-RP | CCAATGATAGGGCGAAAGAA |
| 21 | CAR-FP | CCAGCTCATCTGTTCATCCA |
| 22 | CAR-RP | GGTAACTCCAGGTCGGTCAG |
| 23 | PXR-FP | CAAGCGGAAGAAAAGTGAAC |
| 24 | PXR-RP | CACAGATCTTTCCGGACCTG |