Saturation kinetic parameters for AOX-catalyzed oxidation of phthalazine and DACA in GPLC, RMLC, and human liver cytosol
Each value represents an average ± S.E.M. for triplicate determinations except DACA and human liver cytosol (HLC) which was done in duplicate.
| Vmax | Km | Ki | Vmax/Km | |
|---|---|---|---|---|
| nmol min−1 mg−1 | μM | μM | ml min−1 mg−1 | |
| Phthalazine | ||||
| GPLC | 26.3 ± 0.9 | 4.5 ± 0.7 | NA | 5.8 |
| RMLC | 55.8 ± 2.7 | 3.4 ± 0.8 | NA | 16.4 |
| HLC | 9.2 ± 0.4 | 12.1 ± 1.7 | NA | 0.8 |
| DACA | ||||
| GPLC | 2.2 ± 0.2 | 0.3 ± 0.07 | 3.2 ± 0.8 | 7.3 |
| RMLC | 14.8 ± 3.7 | 8.7 ± 3.3 | 22.32 ± 9.4 | 1.7 |
| HLC | 3.7 ± 0.08 | 8.8 ± 0.4 | 652.2 ± 78.35 | 0.4 |
NA, not applicable.