TABLE 1

Apparent kinetic parameters for the formation of 2-hydroxymetronidazole in human liver microsomes

	Low K_m Site			High K_m Site
Sample	K_m^{^a}	V_max1^{^a}	V_max1/K_m1	K_m2^{^a}	V_max2^{^a}	V_max2/K_m2
	mM	nmol/mg protein  per minute	ml/mg protein  per minute	mM	nmol/mg protein  per minute	ml/mg protein  per minute
H201	0.366 ± 0.029	0.911 ± 0.044	2.48	5.7 ± 4.0	1.30 ± 0.20	0.22
H382	0.527 ± 0.056	0.235 ± 0.017	0.45	23.6 ± 3.4	1.41 ± 0.10	0.06
H469	—	—	—	75.1 ± 10.4	1.44 ± 0.12	0.02
Adult Pool	0.383 ± 0.040	0.213 ± 0.012	0.56	33.1 ± 6.5	0.72 ± 0.10	0.02

↵^a Value ± S.E. of parameter fit.