Metabolites of GLP-1(9-36)amide in mouse and human hepatocytes
| Compounda | tR | Peptide Sequenceb | Mouse Hepatocytes | Human Hepatocytes |
|---|---|---|---|---|
| min | [Monoisotopic (M+H)+1/(M+4H)+4] | |||
| GLP-1(9-36) | 18.67 | EGTFTSDVSSYLEGQAAKEFIAWLVKGR | ||
| 3088.5738/773.8989 | ||||
| M1 | 18.09 | DVSSYLEGQAAKEFIAWLVKGR | Yes | Yes |
| GLP-1(15-36) | 2466.3144/617.3339 | |||
| M2 | 17.99 | FTSDVSSYLEGQAAKEFIAWLVKGR | Yes | Yes |
| GLP-1(12-36) | 2801.4625/701.1210 | |||
| M3 | 17.47 | TSDVSSYLEGQAAKEFIAWLVKGR | Yes | Yes |
| GLP-1(13-36) | 2654.3941/684.3539 | |||
| M4 | 16.36 | VSSYLEGQAAKEFIAWLVKGR | Yes | Yes |
| GLP-1(16-36) | 2351.2875/588.5772 | |||
| M5 | 16.07 | SYLEGQAAKEFIAWLVKGR | Yes | Yes |
| GLP-1(18-36) | 2165.1870/542.0521 | |||
| M6 | 15.78 | YLEGQAAKEFIAWLVKGR | Yes | Yes |
| GLP-1(19-36) | 2078.1550/520.2941 | |||
| M7 | 15.10 | LEGQAAKEFIAWLVKGR | Yes | Yes |
| GLP-1(20-36) | 1915.0917/479.8283 | |||
| M8 | 14.53 | EGQAAKEFIAWLVKGR | Yes | Yes |
| GLP-1(21-36) | 1802.0076/451.5382 | |||
| M9 | 13.99 | GQAAKEFIAWLVKGR | Yes | No |
| GLP-1(22-36) | 1672.9650/419.2591 (M+3H)3+ | |||
| M10 | 13.44 | EFIAWLVKGR | Yes | Yes |
| GLP-1(27-36) | 1217.7158/406.5766 (M+3H)3+ | |||
| M11 | 13.25 | AAKEFIAWLVKGR | Yes | No |
| GLP-1(24-36) | 1487.8850/372.7266 (M+3H)3+ | |||
| M12 | 12.13 | FIAWLVKGR | Yes | No |
| GLP-1(28-36) | 1088.6732/363.5624 (M+3H)3+ | |||
| M13 | 11.09 | KEFIAWLVKGR | Yes | No |
| GLP-1(26-36) | 1345.8108/449.2750 (M+3H)3+ |
↵a Molecular weights correspond to the amidated form of parent and metabolites.
↵b CID spectra of metabolites M1–M13 are provided in Supplemental Figs. 1–13. Theoretical values of fragment ions (generated from the Prospector software) are depicted in the fragment ion assignment, which match the experimental values.