Kinetic parameters of M1 and M2 formation by human tissue preparations
| Enzyme Source | M1 | M2 | ||||
|---|---|---|---|---|---|---|
| Km | Vmax | CLint | Km | Vmax | CLint | |
| μM | nmol/min/mg protein | μl/min/mg protein | μM | nmol/min/mg protein | μl/min/mg protein | |
| Plasma | 274 | 3.36 | 12.3 | — | — | — |
| Plasma + CaCl2a | 297 | 6.03 | 20.3 | — | — | — |
| Microsome | 210 | 6.90 | 32.9 | — | — | — |
| Microsome + CaCl2a | 274 | 8.03 | 29.3 | — | — | — |
| Microsome + NADPH | 280 | 5.32 | 19.0 | 22.8 | 0.13 | 5.83 |
↵a Concentration of CaCl2 was 1 mM.