TABLE 1

Multiple reaction monitoring parameters of peptides selected for targeted analysis of OATP1B1, OATP1B3, OATP2B1, and P-gp

Labeled amino acid residues are shown in bold and italic.

TransporterPeptideResiduesParent Ion
(z = 2)Product Ions(z = 1)Frag (V)CE (eV)
123
OATP1B1 (Peptide 1)NVTGFFQSFK321–330587.9961.4860.5803.212513
NVTGFFQSFK591.9969.5868.5811.212514
OATP1B1 (Peptide 2)YVEQQYGQPSSK362–373707.5766.5418.31152.515522
YVEQQYGQPSSK711.5774.5426.31160.515522
OATP1B3 (Peptide 1)NVTGFFQSLK321–330570.8927.5826.3622.313014
NVTGFFQSLK574.8935.6834.3630.313014
OATP1B3 (Peptide 2)IYNSVFFGR615–623551.8826.5712.5526.613513
IYNSVFFGR556.8836.6635.2536.613513
OATP2B1 (Peptide 1)VLAVTDSPAR314–323514.8816.4846.4745.313013
VLAVTDSPAR519.9826.4856.4755.312513
OATP2B1 (Peptide 2)SSPAVEQQLLVSGPGK687–702799.0712.31026.31155.318020
SSPAVEQQLLVSGPGK803.01163.31034.3715.918020
P-gp (Peptide 1)NTTGALTTR809–817467.8618.3719.4820.512512
NTTGALTTR472.8628.3729.5830.512512
P-gp (Peptide 2)IATEAIENFR896–905582.3749.5678.5979.513016
IATEAIENFR587.3759.5688.5989.513016
  • CE, collision energy; Frag, fragmentor; OATP, organic anion-transporting polypeptide; P-gp, P-glycoprotein.