Enzyme kinetic parameters for DABE hydrolysis in recombinant CES1, CES2, pooled HLS9 fractions, and pooled HIMs
All reactions were run in triplicate and results are presented as mean ± S.D.
| Metabolic Reaction | Km | Vmax | CLint |
|---|---|---|---|
| µM | pmol/min per mg protein | µl/min per mg protein | |
| CES1 | |||
| DABE → M1 | 24.9 ± 2.9 | 676 ± 26 | 27.2 |
| DABE → M2 | N.D. | N.D. | N.D. |
| CES2 | |||
| DABE → M1 | N.D | N.D. | N.D. |
| DABE → M2 | 5.5 ± 0.8 | 71.1 ± 2.4 | 12.9 |
| HLS9 | |||
| DABE → M1 | 33.5 ± 4.1 | 1174 ± 54 | 35.0 |
| DABE → M2 | 15.4 ± 1.9 | 30.8 ± 1.1 | 2.0 |
| HIM | |||
| DABE → M1 | N.D | N.D. | N.D. |
| DABE → M2 | 8.6 ± 0.9 | 207.1 ± 5.7 | 24.1 |
N.D., kinetic parameters not determined due to the low concentration of metabolites detected.