Comparison of solid-phase (C18 column) and acetonitrile extraction for LC-MS/MS detection of arachidonic acid metabolites from liver of a chick embryo treated for 24 hours with TCDD as described in Materials and Methods
Means ± S.E. (n = 3 replicates) are shown in the table.
| Method | EETs | DHETS | 20-HETE | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 5,6- | 8,9- | 11,12- | 14,15- | 5,6- | 8,9- | 11,12- | 14,15- | ||
| ng/g liver | |||||||||
| C18 column | n.d. | 2 ± 0.8 | 2.4 ± 1.4 | n.d. | n.d. | 2.7 ± 0.9 | 4 ± 0.4 | 7 ± 2 | 4.4 ± 1 |
| Acetonitrile | 195 ± 106 | 9 ± 3 | 10 ± 1.7 | 4.2 ± 0.5 | 5.6 ± 0.5 | 39 ± 7 | 133 ± 11 | 288 ± 37 | 35 ± 4 |
n.d., not detected.