Vmax and Km values of the reduction of benzamidoxime by mARC WT and variants
Data are the means ± S.D. of three different incubations of each protein lot.
| mARC Protein | Protein Lots | Vmaxa | Kma |
|---|---|---|---|
| n | nmol BA/min per mg protein | mM BAO | |
| mARC-1 | |||
| mARC-1 | 4 | 105 ± 23 | 0.2 ± 0.1 |
| V96L | 1 | 138 ± 24 | 0.3 ± 0.1 |
| A165T | 2 | 125 ± 28 | 0.4 ± 0.2 |
| M187K | 2 | 104 ± 22 | 0.3 ± 0.1 |
| C246S | 2 | 93 ± 21 | 0.2 ± 0.1 |
| D247H | 2 | 113 ± 18 | 0.3 ± 0.1 |
| M268I | 1 | 94 ± 18 | 0.2 ± 0.1 |
| mARC-2 | |||
| mARC-2 | 3 | 119 ± 34 | 0.4 ± 0.1 |
| G244S | 1 | 84 ± 10 | 0.4 ± 0.1 |
| C245W | 2 | 48 ± 11b | 0.2 ± 0.1 |
BA, benzamidine; BAO, benzamidoxime.
↵a Each mARC protein was incubated in duplicate and measured twice by HPLC. Kinetic parameters were determined as described in Materials and Methods.
↵b Statistical significant decrease of Vmax compared with WT (P < 0.050, ANOVA followed by Dunnett’s post hoc test; Sigma Plot 11.0)