SNP | Primer Sequence | Tm | Fragment Length of PCR Product |
---|---|---|---|
°C | bp | ||
MARC1 | |||
c.493A>G | F: 5′-Bio-AGTGCACGGCCTGGAGATAGAG | 74.0 | 84 |
R: 5′-TAGGGCTGTGACTTCAGGAAGCT | 72.6 | ||
S: 5′-CTGGTTATCCACTGG | 51.7 | ||
c.560T>A | F: 5′-Bio-GCCCAGTGGATAACCAGCTTC | 72.0 | 109 |
R: 5′-TGGGTCGGAACAAGTCTGCTATT | 73.0 | ||
S: 5′-GGACGTCTCGGTCGC | 59.2 | ||
c.736T>A | F: 5′-Bio-CTCAACTCCAGGCTAGAGAAGAA | 68.1 | 107 |
c.739G>C | R: 5′-GATCCAAAGGGGCATAGTGTTAC | 69.7 | |
S: 5′-TGTTACCTCTGCATAGACA | 51.7 | ||
MARC2 | |||
c.730G>A | F: 5′-AGATGCCTCCCTGGTAGATTTGAA | 73.1 | 108 |
R: 5′-Bio-CTTACCTCCTCAAAAGCATCACAG | 70.3 | ||
S: 5′-GCCAAATATTGTGGTGAC | 53.9 | ||
c.730G>A | F: 5′-Bio-TGCCTCCCTGGTAGATTTGAATA | 70.8 | 120 |
c.735T>G | R: 5′-AAAAGCAGTGGGTCGCTTACCTC | 73.7 | |
S: 5′-TACCTCCTCAAAAGCAT | 50.4 |
Bio, biotin; F, forward primer used for PCR; R, reverse primer used for PCR; S, sequencing primer used for pyrosequencing; Tm, melting temperature.