TABLE 1

Primers used for genotyping of four SNPs in MARC1 and two SNPs in MARC2

SNPPrimer SequenceTmFragment Length of PCR Product
°Cbp
MARC1
 c.493A>GF: 5′-Bio-AGTGCACGGCCTGGAGATAGAG74.084
R: 5′-TAGGGCTGTGACTTCAGGAAGCT72.6
S: 5′-CTGGTTATCCACTGG51.7
 c.560T>AF: 5′-Bio-GCCCAGTGGATAACCAGCTTC72.0109
R: 5′-TGGGTCGGAACAAGTCTGCTATT73.0
S: 5′-GGACGTCTCGGTCGC59.2
 c.736T>AF: 5′-Bio-CTCAACTCCAGGCTAGAGAAGAA68.1107
 c.739G>CR: 5′-GATCCAAAGGGGCATAGTGTTAC69.7
S: 5′-TGTTACCTCTGCATAGACA51.7
MARC2
 c.730G>AF: 5′-AGATGCCTCCCTGGTAGATTTGAA73.1108
R: 5′-Bio-CTTACCTCCTCAAAAGCATCACAG70.3
S: 5′-GCCAAATATTGTGGTGAC53.9
 c.730G>AF: 5′-Bio-TGCCTCCCTGGTAGATTTGAATA70.8120
 c.735T>GR: 5′-AAAAGCAGTGGGTCGCTTACCTC73.7
S: 5′-TACCTCCTCAAAAGCAT50.4
  • Bio, biotin; F, forward primer used for PCR; R, reverse primer used for PCR; S, sequencing primer used for pyrosequencing; Tm, melting temperature.