TABLE 2

Fold induction of human CYP2C8, CYP2C9, CYP2C19, and CYP3A4 mRNA in eight lots of cryopreserved hepatocytes and HepaRG cells

P450 mRNAInducerRifampicinPhenobarbital
Lot No.A4A5C4C5C6F1F2F3G4F1F2F3
CYP2C83.02.01.71.10.72.51.61.80.82.41.50.8
CYP2C93.41.31.72.13.62.62.12.51.04.42.81.7
CYP2C191.61.31.41.01.21.20.81.21.11.51.31.4
CYP3A43.13.224.733.424.03.08.613.95.77.57.56.3
  • The eight cryopreserved hepatocyte lots, supplied by three commercial vendors (A, C, and F), and HepaRG (G) were cultured, treated with rifampicin or phenobarbital for 72 h, and used for qPCR analysis, which was carried out as described in Materials and Methods. Values are shown as mean of duplicate.