TABLE 1.

Protein levels of UGT isoforms in biologic specimens

HLMHIM
IsoformIndividualPoolaIndividualPoolbHKMcrhUGT
AvedS.D.%CVAveeS.D.%CV
1A112463.150.913539.621.454.130.6ND3740
1A320.620.699.911.71.93f1.1056.91.30ND1280
1A484.030.436.280.01.60f0.9056.51.40ND826
1A622.612.755.926.4NDND13.91980
1A7NDNDNDNDND1250
1A8NDNDNDNDND4500
1A961.122.236.457.5NDND1711900
1A10NDND17.96.9538.713.1ND1960
2B410247.646.998.8NDNDND883
2B720067.833.915215.79.8162.612.595.32720
2B1069.331.745.749.5NDNDND292
2B1599.742.642.790.8NDNDND878
2B1754.360.811225.511236.632.772.8ND2860
  • Ave, average; ND, not determined because protein levels were below the lower limit of quantification (LLOQ; 1 pmol/mg protein for HLM, and 2 pmol/mg protein for HIM and HKM).

  • a Pooled microsomes of 50 individuals (31 males and 19 females).

  • b Pooled microsomes of 4 individuals (2 males and 2 females).

  • c Pooled microsomes of 18 individuals (11 males and 7 females).

  • d Average value of 16 individuals (9 males and 7 females). The levels of UGT1A6 and UGT2B17 were below the LLOQ in one and five samples, respectively. Values below the LLOQ were treated as zero for calculation.

  • e Average value of 6 individuals (6 males).

  • f Protein levels in four individual samples were below the LLOQ and were extrapolated, because the analyte peak was detected clearly (the signal to noise ratio >5). The average was calculated using extrapolated values.