Protein expression levels of transporters in plasma membrane fraction of humanized liver of PXB mice
Unit of protein expression is pmol/mg protein of plasma membrane fraction.
| Donor | ALL (n = 11) | BD85 (n = 5) | BD72 (n = 3) | BD87 (n = 3) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | S.D. | %CV | n | Mean | S.D. | %CV | n | Mean | S.D. | %CV | n | Mean | S.D. | %CV | n | |
| Canalicular localized transporter | ||||||||||||||||
| MDR3/ABCB4* | 8.68 | 7.32 | 84.3 | 11 | 4.19 | 1.29 | 31 | 5 | 7.47 | 3.66 | 49 | 3 | 17.4 | 9.24 | 53 | 3 |
| BSEP/ABCB11* | 7.57 | 7.91 | 104 | 11 | 3.06 | 1.13 | 37 | 5 | 6.12 | 2.79 | 46 | 3 | 16.5 | 11.29 | 68 | 3 |
| MDR1/ABCB1 | 4.71 | 1.78 | 37.7 | 11 | 4.30 | 1.55 | 36 | 5 | 3.86 | 1.19 | 31 | 3 | 6.26 | 2.10 | 34 | 3 |
| MRP2/ABCC2* | 2.37 | 0.88 | 37.1 | 11 | 2.06 | 0.91 | 44 | 5 | 1.89 | 0.19 | 10 | 3 | 3.36 | 0.34 | 10 | 3 |
| MATE1 | 1.10 | 0.22 | 19.7 | 11 | 1.07 | 0.23 | 22 | 5 | 1.28 | 0.21 | 17 | 3 | 0.981 | 0.086 | 9 | 3 |
| ABCG8* | 0.956 | 0.701 | 73.3 | 11 | 0.417 | 0.106 | 25 | 5 | 1.89 | 0.62 | 33 | 3 | 0.921 | 0.132 | 14 | 3 |
| BCRP/ABCG2 | LLOQ | LLOQ | N.D. | N.D. | ||||||||||||
| γ-GTP | 2.22 | 0.89 | 40.1 | 11 | 2.52 | 0.60 | 24 | 5 | 1.57 | 0.18 | 12 | 3 | 2.39 | 1.52 | 64 | 3 |
| Sinusoidal localized transporter | ||||||||||||||||
| OCT1* | 4.21 | 3.41 | 81.0 | 11 | 6.87 | 3.04 | 44 | 5 | 3.22 | 1.61 | 50 | 3 | 0.765 | 0.102 | 13 | 3 |
| OAT2 | 4.09 | 1.18 | 28.8 | 11 | 4.89 | 1.08 | 22 | 5 | 3.76 | 1.06 | 28 | 3 | 3.09 | 0.49 | 16 | 3 |
| NTCP | 2.42 | 0.81 | 33.5 | 11 | 2.12 | 1.12 | 53 | 5 | 2.61 | 0.34 | 13 | 3 | 2.71 | 0.47 | 17 | 3 |
| MRP6 | 2.29 | 0.51 | 22.2 | 11 | 2.57 | 0.58 | 23 | 5 | 1.97 | 0.48 | 25 | 3 | 2.14 | 0.08 | 4 | 3 |
| OATP1B1 | 1.45 | 0.56 | 38.9 | 11 | 1.22 | 0.63 | 52 | 5 | 1.50 | 0.16 | 11 | 3 | 1.78 | 0.68 | 38 | 3 |
| OATP1B3* | 1.03 | 0.47 | 45.9 | 11 | 0.616 | 0.207 | 34 | 5 | 1.47 | 0.47 | 32 | 3 | 1.28 | 0.06 | 5 | 3 |
| OATP2B1 | 0.578 | 0.241 | 42.5 | 11 | 0.684 | 0.320 | 47 | 5 | 0.500 | 0.119 | 24 | 3 | 0.442 | 0.090 | 20 | 3 |
| Na+/K+ ATPase | 22.6 | 6.65 | 29.4 | 11 | 23.6 | 9.00 | 38 | 5 | 22.0 | 1.7 | 8 | 3 | 21.7 | 7.22 | 33 | 3 |
| Localization unknown | ||||||||||||||||
| ENT1 | 1.55 | 0.50 | 32.6 | 11 | 1.79 | 0.60 | 34 | 5 | 1.59 | 0.32 | 20 | 3 | 1.11 | 0.13 | 11 | 3 |
n, Number of determined samples; LLOQ, lower than the limit of quantification; N.D., not determined; ENT, equilibrative nucleoside transporter; NTCP, sodium/taurocholate cotransporting polypeptide.
* Significant difference (P < 0.05) in protein expression levels among donors (BD85, BD72 and BD87).