TABLE 1.

Proteotypic peptides for CYP3A and CYP4F enzymes.

P450Peptide NameProteotypic Peptide SequenceM+HMRM
m/z
CYP3Aa3A_pep1S131LLSPTFTSGK1411138569.6 → 737.6
3A_IS_pep1bS131LLSPTF(9 13C, 15N)TSGK1411148574.4 → 834.5
CYP3A43A4_pep1E244VTNFLR250879439.9 → 650.5
3A4_IS_pep1E244VTNF(9 13C, 15N)LR250889444.8 → 660.5
CYP3A53A5_pep1D244TINFLSK251938469.5 → 608.4
3A5_IS_pep1D244TINF(9 13C, 15N)LSK251948474.4 → 618.5
CYP4F24F2_pep1S109VINASAAIAPK1201142571.6 → 842.6
4F2_IS_pep1S109VI(6 13C, 15N)NASAAIAPK1201149575.0 → 962.6
4F2_pep2V480VLALTLLR488998499.6 → 799.7
4F2_IS_pep2V480VL(6 13C, 15N)ALTLLR4881005503.0 → 806.7
CYP4F3B4F3B_pep1S109VINASAAIVPK1201170585.6 → 870.6
4F3B_IS_pep1S109VI(6 13C, 15N)NASAAIVPK1201177589.0 → 870.6
4F3B_pep2V480VLGLTLLR488984492.5 → 785.6
4F3B_IS_pep2V480VL(6 13C, 15N)GLTLLR488991496.0 → 792.6

  • ↵a This peptide detects CYP3A4, CYP3A5, and CYP3A7, but not CYP3A43.

  • ↵b The 13C- and 15N-labeled peptides served as internal standards during quantitation.