TABLE 1

Effects of flavonoids on the uptake of [14C]PAH in both OAT1-expressing and OAT1-negative LLC-PK1 cells

The uptake of [14C]PAH (0.5 μM) in both OAT1-expressing and OAT1-negative LLC-PK1 cells in the absence or presence of flavonoids (50 μM) was performed as described in Materials and Methods. Probenecid (200 μM) was used as a positive control. The uptake of [14C]PAH is expressed as the percentage of control (uptake in OAT1-negative cells). The data are expressed as mean ± S.D.; n = 4. The values in the “Change in Mean” columns represent the percentage of decrease or increase in means relative to the control value for either OAT1-expressing or OAT1-negative cells.

FlavonoidsChemical Structure
OAT1-Expressing CellsOAT1-Negative Cells
Mean ± S.D.Change in MeanMean ± S.D.Change in Mean
Percentage
Control2714 ± 77.2100 ± 2.3
Probenecid124 ± 4.42***−95.4109 ± 5.23+9.4
Biochanin AEmbedded Image894 ± 19.6***−67.1125 ± 10.9***+24.9
ChrysinEmbedded Image480 ± 22.4***−82.3121 ± 7.33***+20.5
FisetinEmbedded Image172 ± 7.02***−93.6125 ± 7.63***+24.9
GalanginEmbedded Image1258 ± 109***−53.7106 ± 14.6+6.4
LuteolinEmbedded Image142 ± 5.01***−94.8112 ± 4.32+11.8
MorinEmbedded Image210 ± 7.69***−92.3127 ± 10.4***+27.3
MyricetinEmbedded Image351 ± 8.91***−87.1113 ± 9.78+13.3
SilymarinEmbedded Image525 ± 39.7***−80.7115 ± 11.4+14.8

  • ↵*** P < 0.001 versus the control of either OAT1-expressing or OAT1-negative cells.