TABLE 3

Enzyme kinetic parameters for the formation of major metabolites of CYP1A1, CYP1A2, CYP2C6, and CYP2C11

Data are the mean and S.E.E. (standard error of estimate), V_max (in picomoles per picomoles of P450 per minute), K_m and K_si (in micromolars), and h were determined as per (eq. 4). Cl_int [intrinsic clearance (in microliters per minute per picomoles P450)] was calculated. as V_max/K_m.

Compound		V_max	K_m	K_si	h	Cl_int	R²
CYP1A1	16-HETE	1.79 ± 0.36	41.6 ± 9.32	132 ± 15	2.46 ± 0.61	0.04	0.88
	17-HETE	2.39 ± 0.45	51.6 ± 10.5	132 ± 15	2.30 ± 0.44	0.05	0.96
	18-HETE	3.77 ± 0.60	48.3 ± 7.32	132 ± 15	2.37 ± 0.32	0.08	0.96
	19-HETE	7.75 ± 1.04	39.5 ± 4.77	132 ± 15	2.27 ± 0.22	0.20	0.96
CYP1A2	16-HETE	2.18 ± 0.13	10.1 ± 0.82	121 ± 5.33	2.71 ± 0.44	0.22	0.98
	17-HETE	0.44 ± 0.14	11.3 ± 6.53	121 ± 5.33	2.00 ± 0.55	0.04	0.99
	18-HETE	0.73 ± 0.11	9.92 ± 2.31	121 ± 5.33	2.71 ± 1.41	0.07	0.98
	19-HETE	1.17 ± 0.11	9.76 ± 1.26	121 ± 5.33	3.00 ± 1.00	0.12	0.98
CYP2C6	12-HETE	2.65 ± 0.29	18.3 ± 2.84	142 ± 5.91	2.39 ± 0.55	0.14	0.96
	15-HETE	0.82 ± 0.13	13 ± 2.02	142 ± 5.91	5.40 ± 2.33	0.06	0.93
	11,12-EET	1.49 ± 0.40	35.9 ± 18.5	142 ± 5.91	1.44 ± 0.54	0.04	0.97
	14,15-EET	2.10 ± 0.39	31.9 ± 11.1	142 ± 5.91	1.46 ± 0.38	0.07	0.97
CYP2C11	5,6-EET	4.99 ± 1.26	90.2 ± 43.7	82.9 ± 5.05	1.33 ± 0.42	0.06	0.90
	8,9-EET	8.36 ± 1.25	65.6 ± 10.6	82.9 ± 5.05	2.13 ± 0.29	0.13	0.97
	11,12-EET	12 ± 1.38	60.1 ± 6.78	82.9 ± 5.05	2.18 ± 0.20	0.20	0.96
	14,15-EET	7.77 ± 1.14	62.3 ± 11.2	82.9 ± 5.05	1.89 ± 0.28	0.12	0.98