TABLE 3

Average parameter estimates (n = 100 repeats) for time-dependent inhibition with Michaelis-Menten kinetics

Data were simulated with the following parameters: K_I = 10 µM, k_inact = 0.025 min⁻¹.

Model (% Error in Simulated Data)	Parameter	Numerical Method^{^a}	Replot Method^{^a}
MM (10%)	K_I	8.6–10.8	3.9–28.5
	k_inact	0.020–0.028	0.019–0.034
	k_inact/K_I	0.0011–0.0056	0.0013–0.0073
MM (5%)	K_I	9.4–10.4	6.7–16.4
	k_inact	0.023–0.027	0.023–0.029
	k_inact/K_I	0.0024–0.0026	0.0017–0.0040
MM (2.5%)	K_I	9.7–10.3	7.6–12.1
	k_inact	0.024–0.026	0.024–0.027
	k_inact/K_I	0.0024–0.0026	0.0020–0.0032

MM, Michaelis-Menten.
↵^a Data are represented as a range of ±1 S.D. determined from the log normal distribution of 100 runs.