TABLE 1

An example of raw data for in vitro TDI analyses

One repeat of dataset 4 is listed. TDI is a proprietary compound, and is a CYP3A4 inactivator. Midazolam was used as the substrate. Data are presented as concentration of metabolite formed (μM) at the end of a 6-minute incubation, with a 20-fold dilution upon addition of substrate at the end of the primary incubation time. The analysis of this dataset is provided in Table 5.

Primary Incubation Time	TDI Conc. before Dilution:
Primary Incubation Time	0 µM (Solvent Control)	0.391 µM	0.781 µM	1.56 µM	3.13 µM	6.25 µM	12.5 µM	25 µM	50 µM	100 µM
min	Conc. of metabolite, µM
0	0.095	0.095	0.095	0.096	0.096	0.093	0.092	0.086	0.076	0.063
1	0.090	0.093	0.091	0.092	0.087	0.086	0.088	0.085	0.077	0.058
2	0.091	0.091	0.090	0.087	0.085	0.087	0.082	0.078	0.068	0.055
3	0.088	0.086	0.087	0.087	0.085	0.086	0.081	0.074	0.067	0.055
10	0.084	0.082	0.080	0.078	0.081	0.080	0.075	0.071	0.059	0.045
20	0.080	0.078	0.078	0.079	0.077	0.070	0.068	0.060	0.046	0.034
30	0.080	0.077	0.079	0.076	0.073	0.070	0.065	0.057	0.041	0.030